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Class: SamplePreparation

A process that prepares a sample for imaging

URI: lambdaber:SamplePreparation

classDiagram class SamplePreparation click SamplePreparation href "../SamplePreparation/" NamedThing <|-- SamplePreparation click NamedThing href "../NamedThing/" SamplePreparation : affinity_column SamplePreparation : affinity_type SamplePreparation : aggregation_assessment SamplePreparation : aliquoting SamplePreparation : antibiotic_selection SamplePreparation : cleavage_temperature_c SamplePreparation : cleavage_time_h SamplePreparation : concentration_method SamplePreparation : culture_volume_l SamplePreparation : description SamplePreparation : elution_buffer SamplePreparation : expression_system SamplePreparation --> "0..1" ExpressionSystemEnum : expression_system click ExpressionSystemEnum href "../ExpressionSystemEnum/" SamplePreparation : final_buffer SamplePreparation : final_concentration_mg_per_ml SamplePreparation : growth_temperature_c SamplePreparation : harvest_timepoint SamplePreparation : hic_column SamplePreparation : host_strain_or_cell_line SamplePreparation : id SamplePreparation : iex_column SamplePreparation : inducer_concentration SamplePreparation : induction_agent SamplePreparation : induction_temperature_c SamplePreparation : induction_time_h SamplePreparation : lysis_buffer SamplePreparation : lysis_method SamplePreparation : medium SamplePreparation : od600_at_induction SamplePreparation : operator_id SamplePreparation : preparation_date SamplePreparation : preparation_type SamplePreparation --> "1" PreparationTypeEnum : preparation_type click PreparationTypeEnum href "../PreparationTypeEnum/" SamplePreparation : protease SamplePreparation : protease_inhibitors SamplePreparation : protease_ratio SamplePreparation : protocol_description SamplePreparation : purification_steps SamplePreparation --> "*" PurificationStepEnum : purification_steps click PurificationStepEnum href "../PurificationStepEnum/" SamplePreparation : purity_by_sds_page_percent SamplePreparation : sample_id SamplePreparation : sec_buffer SamplePreparation : sec_column SamplePreparation : second_affinity_reverse SamplePreparation : tag_removal SamplePreparation : title SamplePreparation : wash_buffer SamplePreparation : yield_mg

Inheritance

Slots

Name Cardinality and Range Description Inheritance
preparation_type 1
PreparationTypeEnum		 Type of sample preparation direct
sample_id 1
String		 Reference to the sample being prepared direct
preparation_date 0..1
String		 Date of sample preparation direct
operator_id 0..1
String		 Identifier or name of the person who performed the sample preparation (e direct
protocol_description 0..1
String		 Detailed protocol description direct
expression_system 0..1
ExpressionSystemEnum		 Expression system used for recombinant protein production direct
host_strain_or_cell_line 0..1
String		 Specific strain or cell line used (e direct
culture_volume_l 0..1
Float		 Culture volume in liters direct
medium 0..1
String		 Growth medium used direct
antibiotic_selection 0..1
String		 Antibiotic or selection agent used direct
growth_temperature_c 0..1
Float		 Growth temperature in Celsius direct
induction_agent 0..1
String		 Agent used to induce expression (e direct
inducer_concentration 0..1
String		 Concentration of induction agent direct
induction_temperature_c 0..1
Float		 Temperature during induction in Celsius direct
induction_time_h 0..1
Float		 Duration of induction in hours direct
od600_at_induction 0..1
Float		 Optical density at 600nm when induction was started direct
harvest_timepoint 0..1
String		 Time point when cells were harvested direct
lysis_method 0..1
String		 Method used for cell lysis direct
protease_inhibitors 0..1
String		 Protease inhibitors added direct
purification_steps *
PurificationStepEnum		 Ordered list of purification steps performed direct
affinity_type 0..1
String		 Type of affinity chromatography direct
affinity_column 0..1
String		 Affinity column specifications direct
lysis_buffer 0..1
String		 Buffer composition for lysis direct
wash_buffer 0..1
String		 Buffer composition for washing direct
elution_buffer 0..1
String		 Buffer composition for elution direct
tag_removal 0..1
Boolean		 Whether and how affinity tag was removed direct
protease 0..1
String		 Protease used for tag cleavage direct
protease_ratio 0..1
String		 Ratio of protease to protein direct
cleavage_time_h 0..1
Float		 Duration of protease cleavage in hours direct
cleavage_temperature_c 0..1
Float		 Temperature during cleavage in Celsius direct
second_affinity_reverse 0..1
String		 Second affinity or reverse affinity step direct
iex_column 0..1
String		 Ion-exchange column used direct
hic_column 0..1
String		 Hydrophobic interaction column used direct
sec_column 0..1
String		 Size-exclusion column used direct
sec_buffer 0..1
String		 Buffer for size-exclusion chromatography direct
concentration_method 0..1
String		 Method used to concentrate protein direct
final_buffer 0..1
String		 Final buffer composition after purification direct
final_concentration_mg_per_ml 0..1
Float		 Final protein concentration in mg/mL direct
yield_mg 0..1
Float		 Total yield in milligrams direct
purity_by_sds_page_percent 0..1
Float		 Purity percentage by SDS-PAGE direct
aggregation_assessment 0..1
String		 Assessment of protein aggregation state direct
aliquoting 0..1
String		 How the protein was aliquoted for storage direct
id 1
Uriorcurie		 Globally unique identifier as an IRI or CURIE for machine processing and exte... NamedThing
title 0..1
String		 A human-readable name or title for this entity NamedThing
description 0..1
String		 A detailed textual description of this entity NamedThing

Usages

used by used in type used
Study sample_preparations range SamplePreparation

Identifier and Mapping Information

Schema Source

  • from schema: https://w3id.org/lambda-ber-schema/

Mappings

Mapping Type Mapped Value
self lambdaber:SamplePreparation
native lambdaber:SamplePreparation

LinkML Source

Direct

name: SamplePreparation
description: A process that prepares a sample for imaging
from_schema: https://w3id.org/lambda-ber-schema/
is_a: NamedThing
attributes:
  preparation_type:
    name: preparation_type
    description: Type of sample preparation
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: PreparationTypeEnum
    required: true
  sample_id:
    name: sample_id
    description: Reference to the sample being prepared
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    - ExperimentRun
    range: string
    required: true
  preparation_date:
    name: preparation_date
    description: Date of sample preparation
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  operator_id:
    name: operator_id
    description: Identifier or name of the person who performed the sample preparation
      (e.g., 'jsmith', 'John Smith', or personnel ID)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    - ExperimentRun
    range: string
  protocol_description:
    name: protocol_description
    description: Detailed protocol description
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  expression_system:
    name: expression_system
    description: Expression system used for recombinant protein production
    from_schema: https://w3id.org/lambda-ber-schema/
    domain_of:
    - Sample
    - SamplePreparation
    range: ExpressionSystemEnum
  host_strain_or_cell_line:
    name: host_strain_or_cell_line
    description: Specific strain or cell line used (e.g., BL21(DE3), Sf9, HEK293F)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  culture_volume_l:
    name: culture_volume_l
    description: Culture volume in liters
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
    unit:
      ucum_code: L
  medium:
    name: medium
    description: Growth medium used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  antibiotic_selection:
    name: antibiotic_selection
    description: Antibiotic or selection agent used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  growth_temperature_c:
    name: growth_temperature_c
    description: Growth temperature in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
    unit:
      ucum_code: Cel
  induction_agent:
    name: induction_agent
    description: Agent used to induce expression (e.g., IPTG, tetracycline)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  inducer_concentration:
    name: inducer_concentration
    description: Concentration of induction agent
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  induction_temperature_c:
    name: induction_temperature_c
    description: Temperature during induction in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  induction_time_h:
    name: induction_time_h
    description: Duration of induction in hours
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  od600_at_induction:
    name: od600_at_induction
    description: Optical density at 600nm when induction was started
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  harvest_timepoint:
    name: harvest_timepoint
    description: Time point when cells were harvested
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  lysis_method:
    name: lysis_method
    description: Method used for cell lysis
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  protease_inhibitors:
    name: protease_inhibitors
    description: Protease inhibitors added
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  purification_steps:
    name: purification_steps
    description: Ordered list of purification steps performed
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: PurificationStepEnum
    multivalued: true
  affinity_type:
    name: affinity_type
    description: Type of affinity chromatography
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  affinity_column:
    name: affinity_column
    description: Affinity column specifications
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  lysis_buffer:
    name: lysis_buffer
    description: Buffer composition for lysis
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  wash_buffer:
    name: wash_buffer
    description: Buffer composition for washing
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  elution_buffer:
    name: elution_buffer
    description: Buffer composition for elution
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  tag_removal:
    name: tag_removal
    description: Whether and how affinity tag was removed
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: boolean
  protease:
    name: protease
    description: Protease used for tag cleavage
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  protease_ratio:
    name: protease_ratio
    description: Ratio of protease to protein
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  cleavage_time_h:
    name: cleavage_time_h
    description: Duration of protease cleavage in hours
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  cleavage_temperature_c:
    name: cleavage_temperature_c
    description: Temperature during cleavage in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  second_affinity_reverse:
    name: second_affinity_reverse
    description: Second affinity or reverse affinity step
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  iex_column:
    name: iex_column
    description: Ion-exchange column used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  hic_column:
    name: hic_column
    description: Hydrophobic interaction column used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  sec_column:
    name: sec_column
    description: Size-exclusion column used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  sec_buffer:
    name: sec_buffer
    description: Buffer for size-exclusion chromatography
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  concentration_method:
    name: concentration_method
    description: Method used to concentrate protein
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  final_buffer:
    name: final_buffer
    description: Final buffer composition after purification
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  final_concentration_mg_per_ml:
    name: final_concentration_mg_per_ml
    description: Final protein concentration in mg/mL
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  yield_mg:
    name: yield_mg
    description: Total yield in milligrams
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  purity_by_sds_page_percent:
    name: purity_by_sds_page_percent
    description: Purity percentage by SDS-PAGE
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  aggregation_assessment:
    name: aggregation_assessment
    description: Assessment of protein aggregation state
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  aliquoting:
    name: aliquoting
    description: How the protein was aliquoted for storage
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation

Induced

name: SamplePreparation
description: A process that prepares a sample for imaging
from_schema: https://w3id.org/lambda-ber-schema/
is_a: NamedThing
attributes:
  preparation_type:
    name: preparation_type
    description: Type of sample preparation
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: preparation_type
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: PreparationTypeEnum
    required: true
  sample_id:
    name: sample_id
    description: Reference to the sample being prepared
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: sample_id
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    - ExperimentRun
    range: string
    required: true
  preparation_date:
    name: preparation_date
    description: Date of sample preparation
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: preparation_date
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  operator_id:
    name: operator_id
    description: Identifier or name of the person who performed the sample preparation
      (e.g., 'jsmith', 'John Smith', or personnel ID)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: operator_id
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    - ExperimentRun
    range: string
  protocol_description:
    name: protocol_description
    description: Detailed protocol description
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: protocol_description
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  expression_system:
    name: expression_system
    description: Expression system used for recombinant protein production
    from_schema: https://w3id.org/lambda-ber-schema/
    alias: expression_system
    owner: SamplePreparation
    domain_of:
    - Sample
    - SamplePreparation
    range: ExpressionSystemEnum
  host_strain_or_cell_line:
    name: host_strain_or_cell_line
    description: Specific strain or cell line used (e.g., BL21(DE3), Sf9, HEK293F)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: host_strain_or_cell_line
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  culture_volume_l:
    name: culture_volume_l
    description: Culture volume in liters
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: culture_volume_l
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
    unit:
      ucum_code: L
  medium:
    name: medium
    description: Growth medium used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: medium
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  antibiotic_selection:
    name: antibiotic_selection
    description: Antibiotic or selection agent used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: antibiotic_selection
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  growth_temperature_c:
    name: growth_temperature_c
    description: Growth temperature in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: growth_temperature_c
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
    unit:
      ucum_code: Cel
  induction_agent:
    name: induction_agent
    description: Agent used to induce expression (e.g., IPTG, tetracycline)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: induction_agent
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  inducer_concentration:
    name: inducer_concentration
    description: Concentration of induction agent
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: inducer_concentration
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  induction_temperature_c:
    name: induction_temperature_c
    description: Temperature during induction in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: induction_temperature_c
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  induction_time_h:
    name: induction_time_h
    description: Duration of induction in hours
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: induction_time_h
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  od600_at_induction:
    name: od600_at_induction
    description: Optical density at 600nm when induction was started
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: od600_at_induction
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  harvest_timepoint:
    name: harvest_timepoint
    description: Time point when cells were harvested
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: harvest_timepoint
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  lysis_method:
    name: lysis_method
    description: Method used for cell lysis
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: lysis_method
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  protease_inhibitors:
    name: protease_inhibitors
    description: Protease inhibitors added
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: protease_inhibitors
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  purification_steps:
    name: purification_steps
    description: Ordered list of purification steps performed
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: purification_steps
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: PurificationStepEnum
    multivalued: true
  affinity_type:
    name: affinity_type
    description: Type of affinity chromatography
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: affinity_type
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  affinity_column:
    name: affinity_column
    description: Affinity column specifications
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: affinity_column
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  lysis_buffer:
    name: lysis_buffer
    description: Buffer composition for lysis
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: lysis_buffer
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  wash_buffer:
    name: wash_buffer
    description: Buffer composition for washing
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: wash_buffer
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  elution_buffer:
    name: elution_buffer
    description: Buffer composition for elution
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: elution_buffer
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  tag_removal:
    name: tag_removal
    description: Whether and how affinity tag was removed
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: tag_removal
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: boolean
  protease:
    name: protease
    description: Protease used for tag cleavage
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: protease
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  protease_ratio:
    name: protease_ratio
    description: Ratio of protease to protein
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: protease_ratio
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  cleavage_time_h:
    name: cleavage_time_h
    description: Duration of protease cleavage in hours
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: cleavage_time_h
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  cleavage_temperature_c:
    name: cleavage_temperature_c
    description: Temperature during cleavage in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: cleavage_temperature_c
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  second_affinity_reverse:
    name: second_affinity_reverse
    description: Second affinity or reverse affinity step
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: second_affinity_reverse
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  iex_column:
    name: iex_column
    description: Ion-exchange column used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: iex_column
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  hic_column:
    name: hic_column
    description: Hydrophobic interaction column used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: hic_column
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  sec_column:
    name: sec_column
    description: Size-exclusion column used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: sec_column
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  sec_buffer:
    name: sec_buffer
    description: Buffer for size-exclusion chromatography
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: sec_buffer
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  concentration_method:
    name: concentration_method
    description: Method used to concentrate protein
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: concentration_method
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  final_buffer:
    name: final_buffer
    description: Final buffer composition after purification
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: final_buffer
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  final_concentration_mg_per_ml:
    name: final_concentration_mg_per_ml
    description: Final protein concentration in mg/mL
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: final_concentration_mg_per_ml
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  yield_mg:
    name: yield_mg
    description: Total yield in milligrams
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: yield_mg
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  purity_by_sds_page_percent:
    name: purity_by_sds_page_percent
    description: Purity percentage by SDS-PAGE
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: purity_by_sds_page_percent
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  aggregation_assessment:
    name: aggregation_assessment
    description: Assessment of protein aggregation state
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: aggregation_assessment
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  aliquoting:
    name: aliquoting
    description: How the protein was aliquoted for storage
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: aliquoting
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  id:
    name: id
    description: Globally unique identifier as an IRI or CURIE for machine processing
      and external references. Used for linking data across systems and semantic web
      integration.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    identifier: true
    alias: id
    owner: SamplePreparation
    domain_of:
    - NamedThing
    range: uriorcurie
    required: true
  title:
    name: title
    description: A human-readable name or title for this entity
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    slot_uri: dcterms:title
    alias: title
    owner: SamplePreparation
    domain_of:
    - NamedThing
    range: string
  description:
    name: description
    description: A detailed textual description of this entity
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: description
    owner: SamplePreparation
    domain_of:
    - NamedThing
    - AttributeGroup
    range: string