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Class: CryoEMPreparation

Cryo-EM specific sample preparation

URI: lambdaber:CryoEMPreparation

classDiagram class CryoEMPreparation click CryoEMPreparation href "../CryoEMPreparation/" TechniqueSpecificPreparation <|-- CryoEMPreparation click TechniqueSpecificPreparation href "../TechniqueSpecificPreparation/" CryoEMPreparation : blot_force CryoEMPreparation : blot_number CryoEMPreparation : blot_time CryoEMPreparation : blotter_height CryoEMPreparation : blotter_setting CryoEMPreparation : chamber_temperature CryoEMPreparation : description CryoEMPreparation : ethane_temperature CryoEMPreparation : glow_discharge_applied CryoEMPreparation : glow_discharge_atmosphere CryoEMPreparation : glow_discharge_current CryoEMPreparation : glow_discharge_pressure CryoEMPreparation : glow_discharge_time CryoEMPreparation : grid_material CryoEMPreparation --> "0..1" GridMaterialEnum : grid_material click GridMaterialEnum href "../GridMaterialEnum/" CryoEMPreparation : grid_type CryoEMPreparation --> "0..1" GridTypeEnum : grid_type click GridTypeEnum href "../GridTypeEnum/" CryoEMPreparation : hole_size CryoEMPreparation : humidity_percentage CryoEMPreparation : plasma_treatment CryoEMPreparation : sample_applied_volume CryoEMPreparation : support_film CryoEMPreparation : vitrification_instrument CryoEMPreparation : vitrification_method CryoEMPreparation --> "0..1" VitrificationMethodEnum : vitrification_method click VitrificationMethodEnum href "../VitrificationMethodEnum/" CryoEMPreparation : wait_time

Inheritance

Slots

Name Cardinality and Range Description Inheritance
grid_type 0..1
GridTypeEnum		 Type of EM grid used direct
support_film 0..1
String		 Support film type direct
hole_size 0..1
Float		 Hole size in micrometers (range: 0 direct
vitrification_method 0..1
VitrificationMethodEnum		 Method used for vitrification direct
blot_time 0..1
Float		 Blotting time in seconds (range: 0 direct
blot_force 0..1
Integer		 Blotting force setting direct
humidity_percentage 0..1
Float		 Chamber humidity during vitrification (range: 0-100) direct
chamber_temperature 0..1
Float		 Chamber temperature in Celsius direct
grid_material 0..1
GridMaterialEnum		 Grid material direct
glow_discharge_applied 0..1
Boolean		 Whether glow discharge treatment was applied direct
glow_discharge_time 0..1
Float		 Glow discharge time in seconds direct
glow_discharge_current 0..1
Float		 Glow discharge current in milliamperes direct
glow_discharge_atmosphere 0..1
String		 Glow discharge atmosphere (air, amylamine) direct
glow_discharge_pressure 0..1
Float		 Glow discharge pressure in millibar direct
vitrification_instrument 0..1
String		 Vitrification instrument used (e direct
blot_number 0..1
Integer		 Number of blots applied direct
wait_time 0..1
Float		 Wait time before blotting in seconds direct
blotter_height 0..1
Float		 Blotter height setting direct
blotter_setting 0..1
Float		 Blotter setting value direct
sample_applied_volume 0..1
Float		 Volume of sample applied in microliters direct
ethane_temperature 0..1
Float		 Ethane temperature in Celsius direct
plasma_treatment 0..1
String		 Plasma treatment details direct
description 0..1
String		 AttributeGroup

Identifier and Mapping Information

Schema Source

  • from schema: https://w3id.org/lambda-ber-schema/

Mappings

Mapping Type Mapped Value
self lambdaber:CryoEMPreparation
native lambdaber:CryoEMPreparation

LinkML Source

Direct

name: CryoEMPreparation
description: Cryo-EM specific sample preparation
from_schema: https://w3id.org/lambda-ber-schema/
is_a: TechniqueSpecificPreparation
attributes:
  grid_type:
    name: grid_type
    description: Type of EM grid used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: GridTypeEnum
  support_film:
    name: support_film
    description: Support film type
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: string
  hole_size:
    name: hole_size
    description: 'Hole size in micrometers (range: 0.5-5.0)'
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: float
    minimum_value: 0.5
    maximum_value: 5.0
  vitrification_method:
    name: vitrification_method
    description: Method used for vitrification
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: VitrificationMethodEnum
  blot_time:
    name: blot_time
    description: 'Blotting time in seconds (range: 0.5-10.0)'
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: float
    minimum_value: 0.5
    maximum_value: 10.0
  blot_force:
    name: blot_force
    description: Blotting force setting
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: integer
  humidity_percentage:
    name: humidity_percentage
    description: 'Chamber humidity during vitrification (range: 0-100)'
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: float
    minimum_value: 0
    maximum_value: 100
  chamber_temperature:
    name: chamber_temperature
    description: Chamber temperature in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: float
  grid_material:
    name: grid_material
    description: Grid material
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: GridMaterialEnum
  glow_discharge_applied:
    name: glow_discharge_applied
    description: Whether glow discharge treatment was applied
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: boolean
  glow_discharge_time:
    name: glow_discharge_time
    description: Glow discharge time in seconds
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: float
  glow_discharge_current:
    name: glow_discharge_current
    description: Glow discharge current in milliamperes
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: float
  glow_discharge_atmosphere:
    name: glow_discharge_atmosphere
    description: Glow discharge atmosphere (air, amylamine)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: string
  glow_discharge_pressure:
    name: glow_discharge_pressure
    description: Glow discharge pressure in millibar
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: float
  vitrification_instrument:
    name: vitrification_instrument
    description: Vitrification instrument used (e.g., Vitrobot)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: string
  blot_number:
    name: blot_number
    description: Number of blots applied
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: integer
  wait_time:
    name: wait_time
    description: Wait time before blotting in seconds
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: float
  blotter_height:
    name: blotter_height
    description: Blotter height setting
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: float
  blotter_setting:
    name: blotter_setting
    description: Blotter setting value
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: float
  sample_applied_volume:
    name: sample_applied_volume
    description: Volume of sample applied in microliters
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: float
  ethane_temperature:
    name: ethane_temperature
    description: Ethane temperature in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: float
  plasma_treatment:
    name: plasma_treatment
    description: Plasma treatment details
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation

Induced

name: CryoEMPreparation
description: Cryo-EM specific sample preparation
from_schema: https://w3id.org/lambda-ber-schema/
is_a: TechniqueSpecificPreparation
attributes:
  grid_type:
    name: grid_type
    description: Type of EM grid used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: grid_type
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: GridTypeEnum
  support_film:
    name: support_film
    description: Support film type
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: support_film
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: string
  hole_size:
    name: hole_size
    description: 'Hole size in micrometers (range: 0.5-5.0)'
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: hole_size
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: float
    minimum_value: 0.5
    maximum_value: 5.0
  vitrification_method:
    name: vitrification_method
    description: Method used for vitrification
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: vitrification_method
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: VitrificationMethodEnum
  blot_time:
    name: blot_time
    description: 'Blotting time in seconds (range: 0.5-10.0)'
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: blot_time
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: float
    minimum_value: 0.5
    maximum_value: 10.0
  blot_force:
    name: blot_force
    description: Blotting force setting
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: blot_force
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: integer
  humidity_percentage:
    name: humidity_percentage
    description: 'Chamber humidity during vitrification (range: 0-100)'
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: humidity_percentage
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: float
    minimum_value: 0
    maximum_value: 100
  chamber_temperature:
    name: chamber_temperature
    description: Chamber temperature in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: chamber_temperature
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: float
  grid_material:
    name: grid_material
    description: Grid material
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: grid_material
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: GridMaterialEnum
  glow_discharge_applied:
    name: glow_discharge_applied
    description: Whether glow discharge treatment was applied
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: glow_discharge_applied
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: boolean
  glow_discharge_time:
    name: glow_discharge_time
    description: Glow discharge time in seconds
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: glow_discharge_time
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: float
  glow_discharge_current:
    name: glow_discharge_current
    description: Glow discharge current in milliamperes
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: glow_discharge_current
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: float
  glow_discharge_atmosphere:
    name: glow_discharge_atmosphere
    description: Glow discharge atmosphere (air, amylamine)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: glow_discharge_atmosphere
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: string
  glow_discharge_pressure:
    name: glow_discharge_pressure
    description: Glow discharge pressure in millibar
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: glow_discharge_pressure
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: float
  vitrification_instrument:
    name: vitrification_instrument
    description: Vitrification instrument used (e.g., Vitrobot)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: vitrification_instrument
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: string
  blot_number:
    name: blot_number
    description: Number of blots applied
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: blot_number
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: integer
  wait_time:
    name: wait_time
    description: Wait time before blotting in seconds
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: wait_time
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: float
  blotter_height:
    name: blotter_height
    description: Blotter height setting
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: blotter_height
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: float
  blotter_setting:
    name: blotter_setting
    description: Blotter setting value
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: blotter_setting
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: float
  sample_applied_volume:
    name: sample_applied_volume
    description: Volume of sample applied in microliters
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: sample_applied_volume
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: float
  ethane_temperature:
    name: ethane_temperature
    description: Ethane temperature in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: ethane_temperature
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: float
  plasma_treatment:
    name: plasma_treatment
    description: Plasma treatment details
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: plasma_treatment
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: string
  description:
    name: description
    from_schema: https://w3id.org/lambda-ber-schema/
    alias: description
    owner: CryoEMPreparation
    domain_of:
    - NamedThing
    - AttributeGroup
    range: string