Class: CryoEMPreparation

Cryo-EM specific sample preparation

classDiagram class CryoEMPreparation click CryoEMPreparation href "../CryoEMPreparation/" TechniqueSpecificPreparation <|-- CryoEMPreparation click TechniqueSpecificPreparation href "../TechniqueSpecificPreparation/" CryoEMPreparation : blot_force CryoEMPreparation --> "0..1" QuantityValue : blot_force click QuantityValue href "../QuantityValue/" CryoEMPreparation : blot_number CryoEMPreparation --> "0..1" QuantityValue : blot_number click QuantityValue href "../QuantityValue/" CryoEMPreparation : blot_time CryoEMPreparation --> "0..1" QuantityValue : blot_time click QuantityValue href "../QuantityValue/" CryoEMPreparation : blotter_height CryoEMPreparation --> "0..1" QuantityValue : blotter_height click QuantityValue href "../QuantityValue/" CryoEMPreparation : blotter_setting CryoEMPreparation --> "0..1" QuantityValue : blotter_setting click QuantityValue href "../QuantityValue/" CryoEMPreparation : chamber_temperature CryoEMPreparation --> "0..1" QuantityValue : chamber_temperature click QuantityValue href "../QuantityValue/" CryoEMPreparation : description CryoEMPreparation : ethane_temperature CryoEMPreparation --> "0..1" QuantityValue : ethane_temperature click QuantityValue href "../QuantityValue/" CryoEMPreparation : glow_discharge_applied CryoEMPreparation : glow_discharge_atmosphere CryoEMPreparation : glow_discharge_current CryoEMPreparation --> "0..1" QuantityValue : glow_discharge_current click QuantityValue href "../QuantityValue/" CryoEMPreparation : glow_discharge_pressure CryoEMPreparation --> "0..1" QuantityValue : glow_discharge_pressure click QuantityValue href "../QuantityValue/" CryoEMPreparation : glow_discharge_time CryoEMPreparation --> "0..1" QuantityValue : glow_discharge_time click QuantityValue href "../QuantityValue/" CryoEMPreparation : grid_material CryoEMPreparation --> "0..1" GridMaterialEnum : grid_material click GridMaterialEnum href "../GridMaterialEnum/" CryoEMPreparation : grid_type CryoEMPreparation --> "0..1" GridTypeEnum : grid_type click GridTypeEnum href "../GridTypeEnum/" CryoEMPreparation : hole_size CryoEMPreparation --> "0..1" QuantityValue : hole_size click QuantityValue href "../QuantityValue/" CryoEMPreparation : humidity_percentage CryoEMPreparation --> "0..1" QuantityValue : humidity_percentage click QuantityValue href "../QuantityValue/" CryoEMPreparation : plasma_treatment CryoEMPreparation : sample_applied_volume CryoEMPreparation --> "0..1" QuantityValue : sample_applied_volume click QuantityValue href "../QuantityValue/" CryoEMPreparation : support_film CryoEMPreparation : vitrification_instrument CryoEMPreparation : vitrification_method CryoEMPreparation --> "0..1" VitrificationMethodEnum : vitrification_method click VitrificationMethodEnum href "../VitrificationMethodEnum/" CryoEMPreparation : wait_time CryoEMPreparation --> "0..1" QuantityValue : wait_time click QuantityValue href "../QuantityValue/"

Inheritance

AttributeGroup
- TechniqueSpecificPreparation
  - CryoEMPreparation

Slots

Name	Cardinality and Range	Description	Inheritance
grid_type	0..1 GridTypeEnum	Type of EM grid used	direct
support_film	0..1 String	Support film type	direct
hole_size	0..1 QuantityValue	Hole size, typically specified in micrometers (range: 0	direct
vitrification_method	0..1 VitrificationMethodEnum	Method used for vitrification	direct
blot_time	0..1 QuantityValue	Blotting time, typically specified in seconds (range: 0	direct
blot_force	0..1 QuantityValue	Blotting force setting	direct
humidity_percentage	0..1 QuantityValue	Chamber humidity during vitrification (range: 0-100), typically specified as ...	direct
chamber_temperature	0..1 QuantityValue	Chamber temperature, typically specified in degrees Celsius	direct
grid_material	0..1 GridMaterialEnum	Grid material	direct
glow_discharge_applied	0..1 Boolean	Whether glow discharge treatment was applied	direct
glow_discharge_time	0..1 QuantityValue	Glow discharge time, typically specified in seconds	direct
glow_discharge_current	0..1 QuantityValue	Glow discharge current, typically specified in milliamperes	direct
glow_discharge_atmosphere	0..1 String	Glow discharge atmosphere (air, amylamine)	direct
glow_discharge_pressure	0..1 QuantityValue	Glow discharge pressure, typically specified in millibars	direct
vitrification_instrument	0..1 String	Vitrification instrument used (e	direct
blot_number	0..1 QuantityValue	Number of blots applied	direct
wait_time	0..1 QuantityValue	Wait time before blotting, typically specified in seconds	direct
blotter_height	0..1 QuantityValue	Blotter height setting	direct
blotter_setting	0..1 QuantityValue	Blotter setting value	direct
sample_applied_volume	0..1 QuantityValue	Volume of sample applied, typically specified in microliters	direct
ethane_temperature	0..1 QuantityValue	Ethane temperature, typically specified in degrees Celsius	direct
plasma_treatment	0..1 String	Plasma treatment details	direct
description	0..1 String		AttributeGroup

Identifier and Mapping Information

Schema Source

from schema: https://w3id.org/lambda-ber-schema/

Mappings

Mapping Type	Mapped Value
self	lambdaber:CryoEMPreparation
native	lambdaber:CryoEMPreparation

LinkML Source

Direct

name: CryoEMPreparation
description: Cryo-EM specific sample preparation
from_schema: https://w3id.org/lambda-ber-schema/
is_a: TechniqueSpecificPreparation
attributes:
  grid_type:
    name: grid_type
    description: Type of EM grid used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: GridTypeEnum
  support_film:
    name: support_film
    description: Support film type
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: string
  hole_size:
    name: hole_size
    description: 'Hole size, typically specified in micrometers (range: 0.5-5.0).
      Data providers may specify alternative units by including the unit in the QuantityValue.'
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  vitrification_method:
    name: vitrification_method
    description: Method used for vitrification
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: VitrificationMethodEnum
  blot_time:
    name: blot_time
    description: 'Blotting time, typically specified in seconds (range: 0.5-10.0).
      Data providers may specify alternative units by including the unit in the QuantityValue.'
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  blot_force:
    name: blot_force
    description: Blotting force setting
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  humidity_percentage:
    name: humidity_percentage
    description: 'Chamber humidity during vitrification (range: 0-100), typically
      specified as a percentage. Data providers may specify as decimal fraction by
      including the unit in the QuantityValue.'
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  chamber_temperature:
    name: chamber_temperature
    description: Chamber temperature, typically specified in degrees Celsius. Data
      providers may specify alternative units by including the unit in the QuantityValue.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  grid_material:
    name: grid_material
    description: Grid material
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: GridMaterialEnum
  glow_discharge_applied:
    name: glow_discharge_applied
    description: Whether glow discharge treatment was applied
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: boolean
  glow_discharge_time:
    name: glow_discharge_time
    description: Glow discharge time, typically specified in seconds. Data providers
      may specify alternative units by including the unit in the QuantityValue.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  glow_discharge_current:
    name: glow_discharge_current
    description: Glow discharge current, typically specified in milliamperes. Data
      providers may specify alternative units by including the unit in the QuantityValue.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  glow_discharge_atmosphere:
    name: glow_discharge_atmosphere
    description: Glow discharge atmosphere (air, amylamine)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: string
  glow_discharge_pressure:
    name: glow_discharge_pressure
    description: Glow discharge pressure, typically specified in millibars. Data providers
      may specify alternative units by including the unit in the QuantityValue.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  vitrification_instrument:
    name: vitrification_instrument
    description: Vitrification instrument used (e.g., Vitrobot)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: string
  blot_number:
    name: blot_number
    description: Number of blots applied
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  wait_time:
    name: wait_time
    description: Wait time before blotting, typically specified in seconds. Data providers
      may specify alternative units by including the unit in the QuantityValue.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  blotter_height:
    name: blotter_height
    description: Blotter height setting. Data providers may include unit information
      in the QuantityValue if needed.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  blotter_setting:
    name: blotter_setting
    description: Blotter setting value. Data providers may include unit information
      in the QuantityValue if needed.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  sample_applied_volume:
    name: sample_applied_volume
    description: Volume of sample applied, typically specified in microliters. Data
      providers may specify alternative units by including the unit in the QuantityValue.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  ethane_temperature:
    name: ethane_temperature
    description: Ethane temperature, typically specified in degrees Celsius. Data
      providers may specify alternative units by including the unit in the QuantityValue.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  plasma_treatment:
    name: plasma_treatment
    description: Plasma treatment details
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - CryoEMPreparation

Induced

name: CryoEMPreparation
description: Cryo-EM specific sample preparation
from_schema: https://w3id.org/lambda-ber-schema/
is_a: TechniqueSpecificPreparation
attributes:
  grid_type:
    name: grid_type
    description: Type of EM grid used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: grid_type
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: GridTypeEnum
  support_film:
    name: support_film
    description: Support film type
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: support_film
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: string
  hole_size:
    name: hole_size
    description: 'Hole size, typically specified in micrometers (range: 0.5-5.0).
      Data providers may specify alternative units by including the unit in the QuantityValue.'
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: hole_size
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  vitrification_method:
    name: vitrification_method
    description: Method used for vitrification
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: vitrification_method
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: VitrificationMethodEnum
  blot_time:
    name: blot_time
    description: 'Blotting time, typically specified in seconds (range: 0.5-10.0).
      Data providers may specify alternative units by including the unit in the QuantityValue.'
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: blot_time
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  blot_force:
    name: blot_force
    description: Blotting force setting
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: blot_force
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  humidity_percentage:
    name: humidity_percentage
    description: 'Chamber humidity during vitrification (range: 0-100), typically
      specified as a percentage. Data providers may specify as decimal fraction by
      including the unit in the QuantityValue.'
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: humidity_percentage
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  chamber_temperature:
    name: chamber_temperature
    description: Chamber temperature, typically specified in degrees Celsius. Data
      providers may specify alternative units by including the unit in the QuantityValue.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: chamber_temperature
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  grid_material:
    name: grid_material
    description: Grid material
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: grid_material
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: GridMaterialEnum
  glow_discharge_applied:
    name: glow_discharge_applied
    description: Whether glow discharge treatment was applied
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: glow_discharge_applied
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: boolean
  glow_discharge_time:
    name: glow_discharge_time
    description: Glow discharge time, typically specified in seconds. Data providers
      may specify alternative units by including the unit in the QuantityValue.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: glow_discharge_time
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  glow_discharge_current:
    name: glow_discharge_current
    description: Glow discharge current, typically specified in milliamperes. Data
      providers may specify alternative units by including the unit in the QuantityValue.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: glow_discharge_current
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  glow_discharge_atmosphere:
    name: glow_discharge_atmosphere
    description: Glow discharge atmosphere (air, amylamine)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: glow_discharge_atmosphere
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: string
  glow_discharge_pressure:
    name: glow_discharge_pressure
    description: Glow discharge pressure, typically specified in millibars. Data providers
      may specify alternative units by including the unit in the QuantityValue.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: glow_discharge_pressure
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  vitrification_instrument:
    name: vitrification_instrument
    description: Vitrification instrument used (e.g., Vitrobot)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: vitrification_instrument
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: string
  blot_number:
    name: blot_number
    description: Number of blots applied
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: blot_number
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  wait_time:
    name: wait_time
    description: Wait time before blotting, typically specified in seconds. Data providers
      may specify alternative units by including the unit in the QuantityValue.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: wait_time
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  blotter_height:
    name: blotter_height
    description: Blotter height setting. Data providers may include unit information
      in the QuantityValue if needed.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: blotter_height
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  blotter_setting:
    name: blotter_setting
    description: Blotter setting value. Data providers may include unit information
      in the QuantityValue if needed.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: blotter_setting
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  sample_applied_volume:
    name: sample_applied_volume
    description: Volume of sample applied, typically specified in microliters. Data
      providers may specify alternative units by including the unit in the QuantityValue.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: sample_applied_volume
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  ethane_temperature:
    name: ethane_temperature
    description: Ethane temperature, typically specified in degrees Celsius. Data
      providers may specify alternative units by including the unit in the QuantityValue.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: ethane_temperature
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: QuantityValue
    inlined: true
  plasma_treatment:
    name: plasma_treatment
    description: Plasma treatment details
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: plasma_treatment
    owner: CryoEMPreparation
    domain_of:
    - CryoEMPreparation
    range: string
  description:
    name: description
    from_schema: https://w3id.org/lambda-ber-schema/
    alias: description
    owner: CryoEMPreparation
    domain_of:
    - NamedThing
    - AttributeGroup
    range: string