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Class: SamplePreparation

A process that prepares a sample for imaging

URI: lambdaber:SamplePreparation

classDiagram class SamplePreparation click SamplePreparation href "../SamplePreparation/" NamedThing <|-- SamplePreparation click NamedThing href "../NamedThing/" SamplePreparation : affinity_column SamplePreparation : affinity_type SamplePreparation : aggregation_assessment SamplePreparation : aliquoting SamplePreparation : antibiotic_selection SamplePreparation : cleavage_temperature_c SamplePreparation : cleavage_time_h SamplePreparation : concentration_method SamplePreparation : culture_volume_l SamplePreparation : description SamplePreparation : elution_buffer SamplePreparation : expression_system SamplePreparation --> "0..1" ExpressionSystemEnum : expression_system click ExpressionSystemEnum href "../ExpressionSystemEnum/" SamplePreparation : final_buffer SamplePreparation : final_concentration_mg_per_ml SamplePreparation : growth_temperature_c SamplePreparation : harvest_timepoint SamplePreparation : hic_column SamplePreparation : host_strain_or_cell_line SamplePreparation : id SamplePreparation : iex_column SamplePreparation : inducer_concentration SamplePreparation : induction_agent SamplePreparation : induction_temperature_c SamplePreparation : induction_time_h SamplePreparation : lysis_buffer SamplePreparation : lysis_method SamplePreparation : medium SamplePreparation : od600_at_induction SamplePreparation : operator_id SamplePreparation : preparation_date SamplePreparation : preparation_type SamplePreparation --> "1" PreparationTypeEnum : preparation_type click PreparationTypeEnum href "../PreparationTypeEnum/" SamplePreparation : protease SamplePreparation : protease_inhibitors SamplePreparation : protease_ratio SamplePreparation : protocol_description SamplePreparation : purification_steps SamplePreparation --> "*" PurificationStepEnum : purification_steps click PurificationStepEnum href "../PurificationStepEnum/" SamplePreparation : purity_by_sds_page_percent SamplePreparation : sample_id SamplePreparation : sec_buffer SamplePreparation : sec_column SamplePreparation : second_affinity_reverse SamplePreparation : tag_removal SamplePreparation : title SamplePreparation : wash_buffer SamplePreparation : yield_mg

Inheritance

Slots

Name Cardinality and Range Description Inheritance
preparation_type 1
PreparationTypeEnum		 Type of sample preparation direct
sample_id 1
String		 Reference to the sample being prepared direct
preparation_date 0..1
String		 Date of sample preparation direct
operator_id 0..1
String		 Identifier or name of the person who performed the sample preparation (e direct
protocol_description 0..1
String		 Detailed protocol description direct
expression_system 0..1
ExpressionSystemEnum		 Expression system used for recombinant protein production direct
host_strain_or_cell_line 0..1
String		 Specific strain or cell line used (e direct
culture_volume_l 0..1
Float		 Culture volume in liters direct
medium 0..1
String		 Growth medium used direct
antibiotic_selection 0..1
String		 Antibiotic or selection agent used direct
growth_temperature_c 0..1
Float		 Growth temperature in Celsius direct
induction_agent 0..1
String		 Agent used to induce expression (e direct
inducer_concentration 0..1
String		 Concentration of induction agent direct
induction_temperature_c 0..1
Float		 Temperature during induction in Celsius direct
induction_time_h 0..1
Float		 Duration of induction in hours direct
od600_at_induction 0..1
Float		 Optical density at 600nm when induction was started direct
harvest_timepoint 0..1
String		 Time point when cells were harvested direct
lysis_method 0..1
String		 Method used for cell lysis direct
protease_inhibitors 0..1
String		 Protease inhibitors added direct
purification_steps *
PurificationStepEnum		 Ordered list of purification steps performed direct
affinity_type 0..1
String		 Type of affinity chromatography direct
affinity_column 0..1
String		 Affinity column specifications direct
lysis_buffer 0..1
String		 Buffer composition for lysis direct
wash_buffer 0..1
String		 Buffer composition for washing direct
elution_buffer 0..1
String		 Buffer composition for elution direct
tag_removal 0..1
Boolean		 Whether and how affinity tag was removed direct
protease 0..1
String		 Protease used for tag cleavage direct
protease_ratio 0..1
String		 Ratio of protease to protein direct
cleavage_time_h 0..1
Float		 Duration of protease cleavage in hours direct
cleavage_temperature_c 0..1
Float		 Temperature during cleavage in Celsius direct
second_affinity_reverse 0..1
String		 Second affinity or reverse affinity step direct
iex_column 0..1
String		 Ion-exchange column used direct
hic_column 0..1
String		 Hydrophobic interaction column used direct
sec_column 0..1
String		 Size-exclusion column used direct
sec_buffer 0..1
String		 Buffer for size-exclusion chromatography direct
concentration_method 0..1
String		 Method used to concentrate protein direct
final_buffer 0..1
String		 Final buffer composition after purification direct
final_concentration_mg_per_ml 0..1
Float		 Final protein concentration in mg/mL direct
yield_mg 0..1
Float		 Total yield in milligrams direct
purity_by_sds_page_percent 0..1
Float		 Purity percentage by SDS-PAGE direct
aggregation_assessment 0..1
String		 Assessment of protein aggregation state direct
aliquoting 0..1
String		 How the protein was aliquoted for storage direct
id 1
Uriorcurie		 Globally unique identifier as an IRI or CURIE for machine processing and exte... NamedThing
title 0..1
String		 NamedThing
description 0..1
String		 NamedThing

Usages

used by used in type used
Study sample_preparations range SamplePreparation

Identifier and Mapping Information

Schema Source

  • from schema: https://w3id.org/lambda-ber-schema/

Mappings

Mapping Type Mapped Value
self lambdaber:SamplePreparation
native lambdaber:SamplePreparation

LinkML Source

Direct

name: SamplePreparation
description: A process that prepares a sample for imaging
from_schema: https://w3id.org/lambda-ber-schema/
is_a: NamedThing
attributes:
  preparation_type:
    name: preparation_type
    description: Type of sample preparation
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: PreparationTypeEnum
    required: true
  sample_id:
    name: sample_id
    description: Reference to the sample being prepared
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    - ExperimentRun
    range: string
    required: true
  preparation_date:
    name: preparation_date
    description: Date of sample preparation
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: string
  operator_id:
    name: operator_id
    description: Identifier or name of the person who performed the sample preparation
      (e.g., 'jsmith', 'John Smith', or personnel ID)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    - ExperimentRun
    range: string
  protocol_description:
    name: protocol_description
    description: Detailed protocol description
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  expression_system:
    name: expression_system
    description: Expression system used for recombinant protein production
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: ExpressionSystemEnum
  host_strain_or_cell_line:
    name: host_strain_or_cell_line
    description: Specific strain or cell line used (e.g., BL21(DE3), Sf9, HEK293F)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  culture_volume_l:
    name: culture_volume_l
    description: Culture volume in liters
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  medium:
    name: medium
    description: Growth medium used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  antibiotic_selection:
    name: antibiotic_selection
    description: Antibiotic or selection agent used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  growth_temperature_c:
    name: growth_temperature_c
    description: Growth temperature in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  induction_agent:
    name: induction_agent
    description: Agent used to induce expression (e.g., IPTG, tetracycline)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  inducer_concentration:
    name: inducer_concentration
    description: Concentration of induction agent
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  induction_temperature_c:
    name: induction_temperature_c
    description: Temperature during induction in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  induction_time_h:
    name: induction_time_h
    description: Duration of induction in hours
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  od600_at_induction:
    name: od600_at_induction
    description: Optical density at 600nm when induction was started
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  harvest_timepoint:
    name: harvest_timepoint
    description: Time point when cells were harvested
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  lysis_method:
    name: lysis_method
    description: Method used for cell lysis
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  protease_inhibitors:
    name: protease_inhibitors
    description: Protease inhibitors added
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  purification_steps:
    name: purification_steps
    description: Ordered list of purification steps performed
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: PurificationStepEnum
    multivalued: true
  affinity_type:
    name: affinity_type
    description: Type of affinity chromatography
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  affinity_column:
    name: affinity_column
    description: Affinity column specifications
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  lysis_buffer:
    name: lysis_buffer
    description: Buffer composition for lysis
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  wash_buffer:
    name: wash_buffer
    description: Buffer composition for washing
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  elution_buffer:
    name: elution_buffer
    description: Buffer composition for elution
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  tag_removal:
    name: tag_removal
    description: Whether and how affinity tag was removed
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: boolean
  protease:
    name: protease
    description: Protease used for tag cleavage
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  protease_ratio:
    name: protease_ratio
    description: Ratio of protease to protein
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  cleavage_time_h:
    name: cleavage_time_h
    description: Duration of protease cleavage in hours
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  cleavage_temperature_c:
    name: cleavage_temperature_c
    description: Temperature during cleavage in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  second_affinity_reverse:
    name: second_affinity_reverse
    description: Second affinity or reverse affinity step
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  iex_column:
    name: iex_column
    description: Ion-exchange column used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  hic_column:
    name: hic_column
    description: Hydrophobic interaction column used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  sec_column:
    name: sec_column
    description: Size-exclusion column used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  sec_buffer:
    name: sec_buffer
    description: Buffer for size-exclusion chromatography
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  concentration_method:
    name: concentration_method
    description: Method used to concentrate protein
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  final_buffer:
    name: final_buffer
    description: Final buffer composition after purification
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  final_concentration_mg_per_ml:
    name: final_concentration_mg_per_ml
    description: Final protein concentration in mg/mL
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  yield_mg:
    name: yield_mg
    description: Total yield in milligrams
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  purity_by_sds_page_percent:
    name: purity_by_sds_page_percent
    description: Purity percentage by SDS-PAGE
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
    range: float
  aggregation_assessment:
    name: aggregation_assessment
    description: Assessment of protein aggregation state
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation
  aliquoting:
    name: aliquoting
    description: How the protein was aliquoted for storage
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    domain_of:
    - SamplePreparation

Induced

name: SamplePreparation
description: A process that prepares a sample for imaging
from_schema: https://w3id.org/lambda-ber-schema/
is_a: NamedThing
attributes:
  preparation_type:
    name: preparation_type
    description: Type of sample preparation
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: preparation_type
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: PreparationTypeEnum
    required: true
  sample_id:
    name: sample_id
    description: Reference to the sample being prepared
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: sample_id
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    - ExperimentRun
    range: string
    required: true
  preparation_date:
    name: preparation_date
    description: Date of sample preparation
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: preparation_date
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  operator_id:
    name: operator_id
    description: Identifier or name of the person who performed the sample preparation
      (e.g., 'jsmith', 'John Smith', or personnel ID)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: operator_id
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    - ExperimentRun
    range: string
  protocol_description:
    name: protocol_description
    description: Detailed protocol description
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: protocol_description
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  expression_system:
    name: expression_system
    description: Expression system used for recombinant protein production
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: expression_system
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: ExpressionSystemEnum
  host_strain_or_cell_line:
    name: host_strain_or_cell_line
    description: Specific strain or cell line used (e.g., BL21(DE3), Sf9, HEK293F)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: host_strain_or_cell_line
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  culture_volume_l:
    name: culture_volume_l
    description: Culture volume in liters
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: culture_volume_l
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  medium:
    name: medium
    description: Growth medium used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: medium
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  antibiotic_selection:
    name: antibiotic_selection
    description: Antibiotic or selection agent used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: antibiotic_selection
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  growth_temperature_c:
    name: growth_temperature_c
    description: Growth temperature in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: growth_temperature_c
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  induction_agent:
    name: induction_agent
    description: Agent used to induce expression (e.g., IPTG, tetracycline)
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: induction_agent
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  inducer_concentration:
    name: inducer_concentration
    description: Concentration of induction agent
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: inducer_concentration
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  induction_temperature_c:
    name: induction_temperature_c
    description: Temperature during induction in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: induction_temperature_c
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  induction_time_h:
    name: induction_time_h
    description: Duration of induction in hours
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: induction_time_h
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  od600_at_induction:
    name: od600_at_induction
    description: Optical density at 600nm when induction was started
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: od600_at_induction
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  harvest_timepoint:
    name: harvest_timepoint
    description: Time point when cells were harvested
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: harvest_timepoint
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  lysis_method:
    name: lysis_method
    description: Method used for cell lysis
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: lysis_method
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  protease_inhibitors:
    name: protease_inhibitors
    description: Protease inhibitors added
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: protease_inhibitors
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  purification_steps:
    name: purification_steps
    description: Ordered list of purification steps performed
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: purification_steps
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: PurificationStepEnum
    multivalued: true
  affinity_type:
    name: affinity_type
    description: Type of affinity chromatography
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: affinity_type
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  affinity_column:
    name: affinity_column
    description: Affinity column specifications
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: affinity_column
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  lysis_buffer:
    name: lysis_buffer
    description: Buffer composition for lysis
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: lysis_buffer
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  wash_buffer:
    name: wash_buffer
    description: Buffer composition for washing
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: wash_buffer
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  elution_buffer:
    name: elution_buffer
    description: Buffer composition for elution
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: elution_buffer
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  tag_removal:
    name: tag_removal
    description: Whether and how affinity tag was removed
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: tag_removal
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: boolean
  protease:
    name: protease
    description: Protease used for tag cleavage
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: protease
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  protease_ratio:
    name: protease_ratio
    description: Ratio of protease to protein
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: protease_ratio
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  cleavage_time_h:
    name: cleavage_time_h
    description: Duration of protease cleavage in hours
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: cleavage_time_h
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  cleavage_temperature_c:
    name: cleavage_temperature_c
    description: Temperature during cleavage in Celsius
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: cleavage_temperature_c
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  second_affinity_reverse:
    name: second_affinity_reverse
    description: Second affinity or reverse affinity step
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: second_affinity_reverse
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  iex_column:
    name: iex_column
    description: Ion-exchange column used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: iex_column
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  hic_column:
    name: hic_column
    description: Hydrophobic interaction column used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: hic_column
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  sec_column:
    name: sec_column
    description: Size-exclusion column used
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: sec_column
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  sec_buffer:
    name: sec_buffer
    description: Buffer for size-exclusion chromatography
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: sec_buffer
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  concentration_method:
    name: concentration_method
    description: Method used to concentrate protein
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: concentration_method
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  final_buffer:
    name: final_buffer
    description: Final buffer composition after purification
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: final_buffer
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  final_concentration_mg_per_ml:
    name: final_concentration_mg_per_ml
    description: Final protein concentration in mg/mL
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: final_concentration_mg_per_ml
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  yield_mg:
    name: yield_mg
    description: Total yield in milligrams
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: yield_mg
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  purity_by_sds_page_percent:
    name: purity_by_sds_page_percent
    description: Purity percentage by SDS-PAGE
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: purity_by_sds_page_percent
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: float
  aggregation_assessment:
    name: aggregation_assessment
    description: Assessment of protein aggregation state
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: aggregation_assessment
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  aliquoting:
    name: aliquoting
    description: How the protein was aliquoted for storage
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: aliquoting
    owner: SamplePreparation
    domain_of:
    - SamplePreparation
    range: string
  id:
    name: id
    description: Globally unique identifier as an IRI or CURIE for machine processing
      and external references. Used for linking data across systems and semantic web
      integration.
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    identifier: true
    alias: id
    owner: SamplePreparation
    domain_of:
    - NamedThing
    range: uriorcurie
    required: true
  title:
    name: title
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    slot_uri: dcterms:title
    alias: title
    owner: SamplePreparation
    domain_of:
    - NamedThing
    range: string
  description:
    name: description
    from_schema: https://w3id.org/lambda-ber-schema/
    rank: 1000
    alias: description
    owner: SamplePreparation
    domain_of:
    - NamedThing
    - AttributeGroup
    range: string