Enum: PurificationStepEnum

Protein purification steps and methods

Permissible Values

Value	Meaning	Description
affinity_ni_nta	None	Affinity chromatography using Ni-NTA resin
affinity_co_nta	None	Affinity chromatography using Co-NTA resin
affinity_strep	None	Affinity chromatography using Strep-tag
affinity_mbp	None	Affinity chromatography using maltose-binding protein (MBP)
affinity_gst	None	Affinity chromatography using glutathione S-transferase (GST)
tag_cleavage	None	Proteolytic cleavage of purification tags
ion_exchange	None	Ion-exchange chromatography (IEX)
hydrophobic_interaction	None	Hydrophobic interaction chromatography (HIC)
size_exclusion	None	Size-exclusion chromatography (SEC)
dialysis	None	Dialysis or buffer exchange

Slots

Name	Description
purification_steps	Ordered list of purification steps performed

Identifier and Mapping Information

Schema Source

from schema: https://w3id.org/lambda-ber-schema/

LinkML Source

name: PurificationStepEnum
description: Protein purification steps and methods
from_schema: https://w3id.org/lambda-ber-schema/
rank: 1000
permissible_values:
  affinity_ni_nta:
    text: affinity_ni_nta
    description: Affinity chromatography using Ni-NTA resin
  affinity_co_nta:
    text: affinity_co_nta
    description: Affinity chromatography using Co-NTA resin
  affinity_strep:
    text: affinity_strep
    description: Affinity chromatography using Strep-tag
  affinity_mbp:
    text: affinity_mbp
    description: Affinity chromatography using maltose-binding protein (MBP)
  affinity_gst:
    text: affinity_gst
    description: Affinity chromatography using glutathione S-transferase (GST)
  tag_cleavage:
    text: tag_cleavage
    description: Proteolytic cleavage of purification tags
  ion_exchange:
    text: ion_exchange
    description: Ion-exchange chromatography (IEX)
  hydrophobic_interaction:
    text: hydrophobic_interaction
    description: Hydrophobic interaction chromatography (HIC)
  size_exclusion:
    text: size_exclusion
    description: Size-exclusion chromatography (SEC)
  dialysis:
    text: dialysis
    description: Dialysis or buffer exchange